Description
CTRP5, also known as Complement C1q tumor necrosis factor-related protein 5, encodes a short-chain collagen which is strongly expressed in sub-retinal pigment epithelium (sub-RPE), ciliary epithelium and adipose tissue. CTRP5 is increased in mtDNA-depleted myocytes and that it stimulates the phosphorylation of AMP activated protein kinase. CTRP5 plays an important role in the adhesion of the retinal pigment epithelium (RPE) to the Bruch Membrane, and mutations are thought to impair the adhesion, resulting in sub-RPE deposits. Recombinant human CTRP5, fused to His-tag at N-terminals, was expressed in E Coli and purified by conventional chromatography techniques